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mao a  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mao a
    ( A ) Human endometrial assembloids constructed from adult stem cells were treated with expansion medium (ExM) (CTRL) or subjected to hormonal stimulation. Timeline of endometrial assembloid cultured by ExM (CTRL), ovarian steroid hormones simulating secretory phase (SEC), ovarian steroid hormones combining PRL and placental hormones to mimic the window of implantation (WOI). ( B ) Endometrial assembloids from the CTRL, SEC, and WOI groups, which were subjected to hormone treatment on Days 0, 2, and 8, exhibited comparable growth patterns throughout the culture period. Scale bar = 200 μm. ( C ) The dynamic changes of the counts of assembloids over time in each hormone regimen. ( D ) The dynamic changes of the area of assembloids over time in each hormone regimen. ( E ) Heatmap showing receptivity-related gene expression profile of assembloids in each hormone regimen. The color represents log-transformed fold change of gene expression. ( F ) Validation of receptivity markers (IGFBP1, MAOA, and DPP4) with immunofluorescence (IF) in the CTRL, SEC, and WOI endometrial assembloids in vitro. Nuclei were counterstained with DAPI. Scale bar = 30 μm. The bar chart displays the quantitative comparison of receptivity markers among three groups. * p ≤0.05, ** p ≤0.005, *** p ≤0.0005, **** p ≤0.0001.n=4 (CTRL) and 5 (SEC and WOI) (IGFBP1), <t>n=4</t> <t>(MAO-A</t> and DPP4).
    Mao A, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mao+a/MAO-A+Antibody/pmc13043174-15-2-6
    Average 93 stars, based on 81 article reviews
    mao a - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Human receptive endometrial assembloid for deciphering the implantation window"

    Article Title: Human receptive endometrial assembloid for deciphering the implantation window

    Journal: eLife

    doi: 10.7554/eLife.90729

    ( A ) Human endometrial assembloids constructed from adult stem cells were treated with expansion medium (ExM) (CTRL) or subjected to hormonal stimulation. Timeline of endometrial assembloid cultured by ExM (CTRL), ovarian steroid hormones simulating secretory phase (SEC), ovarian steroid hormones combining PRL and placental hormones to mimic the window of implantation (WOI). ( B ) Endometrial assembloids from the CTRL, SEC, and WOI groups, which were subjected to hormone treatment on Days 0, 2, and 8, exhibited comparable growth patterns throughout the culture period. Scale bar = 200 μm. ( C ) The dynamic changes of the counts of assembloids over time in each hormone regimen. ( D ) The dynamic changes of the area of assembloids over time in each hormone regimen. ( E ) Heatmap showing receptivity-related gene expression profile of assembloids in each hormone regimen. The color represents log-transformed fold change of gene expression. ( F ) Validation of receptivity markers (IGFBP1, MAOA, and DPP4) with immunofluorescence (IF) in the CTRL, SEC, and WOI endometrial assembloids in vitro. Nuclei were counterstained with DAPI. Scale bar = 30 μm. The bar chart displays the quantitative comparison of receptivity markers among three groups. * p ≤0.05, ** p ≤0.005, *** p ≤0.0005, **** p ≤0.0001.n=4 (CTRL) and 5 (SEC and WOI) (IGFBP1), n=4 (MAO-A and DPP4).
    Figure Legend Snippet: ( A ) Human endometrial assembloids constructed from adult stem cells were treated with expansion medium (ExM) (CTRL) or subjected to hormonal stimulation. Timeline of endometrial assembloid cultured by ExM (CTRL), ovarian steroid hormones simulating secretory phase (SEC), ovarian steroid hormones combining PRL and placental hormones to mimic the window of implantation (WOI). ( B ) Endometrial assembloids from the CTRL, SEC, and WOI groups, which were subjected to hormone treatment on Days 0, 2, and 8, exhibited comparable growth patterns throughout the culture period. Scale bar = 200 μm. ( C ) The dynamic changes of the counts of assembloids over time in each hormone regimen. ( D ) The dynamic changes of the area of assembloids over time in each hormone regimen. ( E ) Heatmap showing receptivity-related gene expression profile of assembloids in each hormone regimen. The color represents log-transformed fold change of gene expression. ( F ) Validation of receptivity markers (IGFBP1, MAOA, and DPP4) with immunofluorescence (IF) in the CTRL, SEC, and WOI endometrial assembloids in vitro. Nuclei were counterstained with DAPI. Scale bar = 30 μm. The bar chart displays the quantitative comparison of receptivity markers among three groups. * p ≤0.05, ** p ≤0.005, *** p ≤0.0005, **** p ≤0.0001.n=4 (CTRL) and 5 (SEC and WOI) (IGFBP1), n=4 (MAO-A and DPP4).

    Techniques Used: Construct, Cell Culture, Gene Expression, Transformation Assay, Biomarker Discovery, Immunofluorescence, In Vitro, Comparison

    Related Articles

    Immunohistochemical staining:

    Article Title: Profile of 5-HT 2A receptor involved in signaling cascades associated to intracellular inflammation and apoptosis in hepatocytes and its role in carbon tetrachloride-induced hepatotoxicity.
    Article Snippet: Previously, we found that the 5-HT2A receptor plays a key role in cell injury.. However, the mechanism by which the 5-HT2A receptor mediates intracellular processes remains unclear.. In this study, we aimed to clarify this intracellular process in hepatocyte LO2 cells and evaluate its role in CCl4-induced hepatotoxicity in mice.

    Article Title: Carbon tetrachloride induced mitochondrial division, respiratory chain damage, abnormal intracellular [H + ] and apoptosis are due to the activation of 5-HT degradation system in hepatocytes.
    Article Snippet: Previously we found that acute liver injury (ALI) with inflammation caused by carbon tetrachloride (CCl4) was associated with the activation of the 5-HT degradation system (5DS), which includes monoamine oxidase A (MAO-A), the 5-HT2A receptor, and 5-HT synthases in hepatocytes.. This study aimed to determine the role of 5DS in mitochondrial damage and apoptosis.. In hepatocyte LO2 cells, CCl4 activated 5-HT2A receptor at the gene level, and then 5-HT2A receptor mediated the expression of 5-HT synthase and MAO-A at the gene level.

    Staining:

    Article Title: Profile of 5-HT 2A receptor involved in signaling cascades associated to intracellular inflammation and apoptosis in hepatocytes and its role in carbon tetrachloride-induced hepatotoxicity.
    Article Snippet: Previously, we found that the 5-HT2A receptor plays a key role in cell injury.. However, the mechanism by which the 5-HT2A receptor mediates intracellular processes remains unclear.. In this study, we aimed to clarify this intracellular process in hepatocyte LO2 cells and evaluate its role in CCl4-induced hepatotoxicity in mice.

    Article Title: Carbon tetrachloride induced mitochondrial division, respiratory chain damage, abnormal intracellular [H + ] and apoptosis are due to the activation of 5-HT degradation system in hepatocytes.
    Article Snippet: Previously we found that acute liver injury (ALI) with inflammation caused by carbon tetrachloride (CCl4) was associated with the activation of the 5-HT degradation system (5DS), which includes monoamine oxidase A (MAO-A), the 5-HT2A receptor, and 5-HT synthases in hepatocytes.. This study aimed to determine the role of 5DS in mitochondrial damage and apoptosis.. In hepatocyte LO2 cells, CCl4 activated 5-HT2A receptor at the gene level, and then 5-HT2A receptor mediated the expression of 5-HT synthase and MAO-A at the gene level.

    Incubation:

    Article Title: Right versus left ventricular remodeling in heart failure due to chronic volume overload
    Article Snippet: .. Membranes were washed 3 times with PBS-T buffer and then incubated with a primary antibody at room temperature for 2 hours (Annexin V, Abcam, ab141961:1,000; MAO-A, Santa Cruz, sc-271123 - 1:300; NCAM1, Abcam, ab95153, 1:400; Periostin, Abcam, ab92460, 1:1000; TGM2, Millipore, JBC1863304, 1:400). .. The membranes were washed 3 times with PBS-T buffer and then incubated with an appropriate secondary HRP-conjugated antibody diluted 1:10 000 (Anti-Rb IgG Jackson ImmunoResearch, 711-165-152; Anti-Mouse IgG Santa Cruz, sc-2005, 1:10,000 or Anti-Goat IgG Santa Cruz, sc-2354, 1:10,000) at room temperature for 30 minutes.

    other:

    Article Title: Human receptive endometrial assembloid for deciphering the implantation window
    Article Snippet: Antibody , MAO-A (Mouse monoclonal) , Santa Cruz , Cat#: sc-271123 RRID: AB_10609510 , IF (1:50).

    Western Blot:

    Article Title: An immune-sympathetic neuron communication axis guides adipose tissue browning in cancer-associated cachexia
    Article Snippet: SCG neurons were allowed to adhere to the bottom of the chambers for 2 h before the medium was changed to 300 μl pre-warmed L15 Leibovitz full media containing B-27 plus supplement (A3653401, Thermo Fisher Scientific, USA) and maintained at 37 °C in a humidified incubator with 5 % CO2. .. Antibodies used for Western blotting analyses GAPDH (#2118S, 1:50000, Cell signaling, USA), UCP-1 (#14670S, 1:1000, Cell signaling), TH (#MAB318, 1:1000, Millipore, USA), DBH (#ab96615, 1:1000, Abcam, UK), MAO-A (#sc-20156, 1:1000, Santa Cruz, USA), P-HSL (#4126, 1:1000, Cell signaling), HSL (#4107S, 1:1000, Cell signaling), P-STAT3 (#9145S, 1:1000, Cell signaling), STAT3 (#4904S, 1:1000, Cell signaling), FABP4 (#ab81605, 1:1000, Abcam), F4/80 (#ab74383, 1:1000, Abcam), P-ERK (#9102, 1:1000, Cell signaling), ERK (#9101, 1:1000, Cell signaling), P-IRE1 (#ab48187, 1:1000, Abcam), IRE1 (#3294, 1:1000, Cell signaling). .. HRP-linked Anti-rabbit IgG antibody (#A120-201P, 1:8000, Bethyl, USA) and HRP-linked Anti- mouse IgG antibody (#NA 931V, 1:8000, GE healthcare, USA) were used as secondary antibodies.



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    ( A ) Human endometrial assembloids constructed from adult stem cells were treated with expansion medium (ExM) (CTRL) or subjected to hormonal stimulation. Timeline of endometrial assembloid cultured by ExM (CTRL), ovarian steroid hormones simulating secretory phase (SEC), ovarian steroid hormones combining PRL and placental hormones to mimic the window of implantation (WOI). ( B ) Endometrial assembloids from the CTRL, SEC, and WOI groups, which were subjected to hormone treatment on Days 0, 2, and 8, exhibited comparable growth patterns throughout the culture period. Scale bar = 200 μm. ( C ) The dynamic changes of the counts of assembloids over time in each hormone regimen. ( D ) The dynamic changes of the area of assembloids over time in each hormone regimen. ( E ) Heatmap showing receptivity-related gene expression profile of assembloids in each hormone regimen. The color represents log-transformed fold change of gene expression. ( F ) Validation of receptivity markers (IGFBP1, MAOA, and DPP4) with immunofluorescence (IF) in the CTRL, SEC, and WOI endometrial assembloids in vitro. Nuclei were counterstained with DAPI. Scale bar = 30 μm. The bar chart displays the quantitative comparison of receptivity markers among three groups. * p ≤0.05, ** p ≤0.005, *** p ≤0.0005, **** p ≤0.0001.n=4 (CTRL) and 5 (SEC and WOI) (IGFBP1), <t>n=4</t> <t>(MAO-A</t> and DPP4).
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    ( A ) Human endometrial assembloids constructed from adult stem cells were treated with expansion medium (ExM) (CTRL) or subjected to hormonal stimulation. Timeline of endometrial assembloid cultured by ExM (CTRL), ovarian steroid hormones simulating secretory phase (SEC), ovarian steroid hormones combining PRL and placental hormones to mimic the window of implantation (WOI). ( B ) Endometrial assembloids from the CTRL, SEC, and WOI groups, which were subjected to hormone treatment on Days 0, 2, and 8, exhibited comparable growth patterns throughout the culture period. Scale bar = 200 μm. ( C ) The dynamic changes of the counts of assembloids over time in each hormone regimen. ( D ) The dynamic changes of the area of assembloids over time in each hormone regimen. ( E ) Heatmap showing receptivity-related gene expression profile of assembloids in each hormone regimen. The color represents log-transformed fold change of gene expression. ( F ) Validation of receptivity markers (IGFBP1, MAOA, and DPP4) with immunofluorescence (IF) in the CTRL, SEC, and WOI endometrial assembloids in vitro. Nuclei were counterstained with DAPI. Scale bar = 30 μm. The bar chart displays the quantitative comparison of receptivity markers among three groups. * p ≤0.05, ** p ≤0.005, *** p ≤0.0005, **** p ≤0.0001.n=4 (CTRL) and 5 (SEC and WOI) (IGFBP1), <t>n=4</t> <t>(MAO-A</t> and DPP4).
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    Image Search Results


    ( A ) Human endometrial assembloids constructed from adult stem cells were treated with expansion medium (ExM) (CTRL) or subjected to hormonal stimulation. Timeline of endometrial assembloid cultured by ExM (CTRL), ovarian steroid hormones simulating secretory phase (SEC), ovarian steroid hormones combining PRL and placental hormones to mimic the window of implantation (WOI). ( B ) Endometrial assembloids from the CTRL, SEC, and WOI groups, which were subjected to hormone treatment on Days 0, 2, and 8, exhibited comparable growth patterns throughout the culture period. Scale bar = 200 μm. ( C ) The dynamic changes of the counts of assembloids over time in each hormone regimen. ( D ) The dynamic changes of the area of assembloids over time in each hormone regimen. ( E ) Heatmap showing receptivity-related gene expression profile of assembloids in each hormone regimen. The color represents log-transformed fold change of gene expression. ( F ) Validation of receptivity markers (IGFBP1, MAOA, and DPP4) with immunofluorescence (IF) in the CTRL, SEC, and WOI endometrial assembloids in vitro. Nuclei were counterstained with DAPI. Scale bar = 30 μm. The bar chart displays the quantitative comparison of receptivity markers among three groups. * p ≤0.05, ** p ≤0.005, *** p ≤0.0005, **** p ≤0.0001.n=4 (CTRL) and 5 (SEC and WOI) (IGFBP1), n=4 (MAO-A and DPP4).

    Journal: eLife

    Article Title: Human receptive endometrial assembloid for deciphering the implantation window

    doi: 10.7554/eLife.90729

    Figure Lengend Snippet: ( A ) Human endometrial assembloids constructed from adult stem cells were treated with expansion medium (ExM) (CTRL) or subjected to hormonal stimulation. Timeline of endometrial assembloid cultured by ExM (CTRL), ovarian steroid hormones simulating secretory phase (SEC), ovarian steroid hormones combining PRL and placental hormones to mimic the window of implantation (WOI). ( B ) Endometrial assembloids from the CTRL, SEC, and WOI groups, which were subjected to hormone treatment on Days 0, 2, and 8, exhibited comparable growth patterns throughout the culture period. Scale bar = 200 μm. ( C ) The dynamic changes of the counts of assembloids over time in each hormone regimen. ( D ) The dynamic changes of the area of assembloids over time in each hormone regimen. ( E ) Heatmap showing receptivity-related gene expression profile of assembloids in each hormone regimen. The color represents log-transformed fold change of gene expression. ( F ) Validation of receptivity markers (IGFBP1, MAOA, and DPP4) with immunofluorescence (IF) in the CTRL, SEC, and WOI endometrial assembloids in vitro. Nuclei were counterstained with DAPI. Scale bar = 30 μm. The bar chart displays the quantitative comparison of receptivity markers among three groups. * p ≤0.05, ** p ≤0.005, *** p ≤0.0005, **** p ≤0.0001.n=4 (CTRL) and 5 (SEC and WOI) (IGFBP1), n=4 (MAO-A and DPP4).

    Article Snippet: Antibody , MAO-A (Mouse monoclonal) , Santa Cruz , Cat#: sc-271123 RRID: AB_10609510 , IF (1:50).

    Techniques: Construct, Cell Culture, Gene Expression, Transformation Assay, Biomarker Discovery, Immunofluorescence, In Vitro, Comparison